Papers in Press

Papers in the press are accepted peer-reviewed papers published online in manuscript form, but which have not been copyedited and not yet appeared in a regular issue of the journal. Copyediting may lead to small differences between the papers in press version and the final version, therefore please note that titles and content are liable to change during preparation for publication.


Toomas Kramarenko1,2, Kadrin Meremäe1, Jelena Sõgel3, Maiu Kuningas2, Annika Vilem2, Liidia Häkkinen2, Mihkel Mäesaar2, Terje Elias1, Mati Roasto1
OCCURENCE OF ESCHERICHIA COLI O157:H7 IN ESTONIAN DAIRY FARMS AND BEEF PRODUCTION CHAIN IN 2005–2014
In Press
Abstract
OCCURENCE OF ESCHERICHIA COLI O157:H7 IN ESTONIAN DAIRY FARMS AND BEEF PRODUCTION CHAIN IN 2005–2014
Toomas Kramarenko1,2, Kadrin Meremäe1, Jelena Sõgel3, Maiu Kuningas2, Annika Vilem2, Liidia Häkkinen2, Mihkel Mäesaar2, Terje Elias1, Mati Roasto1

1Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Fr. R. Kreutzwaldi 56/3, 51014 Tartu, Estonia
2
Estonian Veterinary and Food Laboratory, Fr. R. Kreutzwaldi 30, 51006 Tartu, Estonia
3
The Veterinary and Food Board, Väike-Paala 3, 11415 Tallinn, Estonia

Escherichia coli O157:H7 human infections are mostly associated with consumption of raw or undercooked beef and raw milk. Ruminants, especially cattle are the main reservoir of the pathogen. The main aim of the present study was to evaluate the occurrence of E. coli O157:H7 in cattle dairy farm and beef production level in Estonia. It was found that E. coli O157:H7 is present at both dairy farm and slaughterhouse level. The occurrence of the pathogen at Estonian dairy farm level was 1.2% from 1312 cattle's sampled. At slaughterhouse level 744 hide swabs were taken from which 30 (4.0%) were positive to E. coli O157:H7. Both stx1 and stx2 genes were determined from isolates and often the combination of both genes was found to be present. Minimal inhibitory concentration was determined for 30 E. coli O157:H7 isolates which revealed 26.7% of multiresistance isolates. In 2009–2010 in total of 188 beef samples were analysed, which all were negative for O157:H7.
Keywords: Escherichia coli O157: H7, cattle, STEC serogroups, virulence markers, drug resistance.
Source: Agraarteadus, 2018, 29(2):In Press
doi: https://dx.doi.org/10.15159/jas.18.09

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HOW TO CITE
Kramarenko, T., Meremäe, K., Sõgel, J., Kuningas, M., Vilem, A., Häkkinen, L., Mäesaar, M., Elias, T., Roasto, M. 2018. Occurence of Eserichia coli O157:H7 in Estonian dairy farms and beef production chain in 2005–2014. Agraarteadus, 29(2):In Presss. doi: 10.15159/jas.18.09 (In Estonian)
AUTHOR CONTACT
1Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Fr. R. Kreutzwaldi 56/3, 51014 Tartu, Estonia
2
Estonian Veterinary and Food Laboratory, Fr. R. Kreutzwaldi 30, 51006 Tartu, Estonia
Highlights: 
  • A total of 1312 cattle’s were tested on the dairy herd level, of which 16 (1.2%) were positive for Escherichia coli O157: H7, and a total of four dairy cattle farm was found to be positive for E. coli O157:H7.
  • At slaughterhouse level 4% (CI95 2.8-5.7%) of the hide samples taken from the brisket area were found to be positive for E. coli O157:H7.
  • Combinations of STEC virulence genes stx2a + eae, stx1a stx2a + eae ja stx2a + eae were determined among isolates obtained from slaughterhouse level, which points to their high potential to cause severe illness in humans.
  • In total of 12 (40%) E. coli O157:H7 isolates from the slaughterhouse level were resistant against one or more antimicrobials.
  • At the meat cutting level, all 188 beef samples tested were negative for E. coli O157: H7.

Aziz Mahdi Abd Al-Shammari1, Mohammed Ali Abood2, Ghassan Jaafar Hamdi1
TECAMIN FLOWER® FOLIAR APPLICATION TO ALLEVIATE WATER DEFICIT EFFECTS ON GROWTH, YIELD AND WATER USE EFFICIENCY OF TOMATO
In Press
Abstract
TECAMIN FLOWER® FOLIAR APPLICATION TO ALLEVIATE WATER DEFICIT EFFECTS ON GROWTH, YIELD AND WATER USE EFFICIENCY OF TOMATO
Aziz Mahdi Abd Al-Shammari1, Mohammed Ali Abood2, Ghassan Jaafar Hamdi1
1Department of Horticulture and Landscape, College of Agriculture, University of Diyala, Iraq
2Department of Soil Science and Water Resources, College of Agriculture, University of Diyala, Iraq

During drought chemical elements in a dry fertilizer go into solution in the soil with difficulty and the nutrients are less available to the plant. Foliar fertilization with Tecamin flower®, could compensate for nutrient deficiency due to water deficit. This study was designed to observe the effects of different application rates of foliar application of Tecamin flower® on growth, yield and field water use efficiency (CWUE) of tomato under water deficit. The field experiment was conducted from March till July 2016 at the teaching and research farm of Diyala University, Iraq. Performance of tomato genotype 'Bobcat', 'Finenss' and 'Hadeer' was assessed by foliar application of Tecamin flower® at (0 and 2.5 mL L-1) and two irrigation levels (50 and 100% based on field capacity). The results showed that chlorophyll content, fruit weight, yield per plant, total yield and CWUE were significantly affected by genotype and irrigations levels and application of Tecamin flower®. 'Bobcat' genotype has the highest value of plant yield (3.39 kg) and total yield (90.19 t ha-1). 'Finenss' has the highest values for number fruit per plant (40.75), CWUE (75.37 kg m-3) and the minimum leaf proline content (2.584 mg g-1). Plant irrigated at 100% had the highest value of number fruit (35.27 fruit), most yield per plant (3.53 kg), total yield (94.02 t ha-1) and minimum leaf proline content (2.557 mg g-1). The highest CWUE (84.50 kg m-3) were at 50% level. Plant treated at 2.5 mL L-1 Tecamin flower® had most fruit/plant (37.61) yield per plant (3.73 kg), total yield (99.16 t ha-1), CWUE (85.22 kg m-3) and minimum leaf proline content (2.437 mg g-1).
Keywords: Solanum lycopersicum, genotype, Foliar application, Tecamin flower®, Water stress.
Source: Agraarteadus, 2018, 29(2):In Press
doi: https://dx.doi.org/10.15159/jas.18.10

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HOW TO CITE
Al-Shammari1, A. M. A., Abood, M. A., Hamdi, G. J. 2018. Tecamin flower® foliar application to alleviate water deficit effects on growth, yield and water use efficiency of tomato. Agraarteadus, 29(2):In Presss. doi: 10.15159/jas.18.10
AUTHOR CONTACT
1Department of Horticulture and Landscape, College of Agriculture, University of Diyala, Iraq
2Department of Soil Science and Water Resources, College of Agriculture, University of Diyala, Iraq

Highlights: 

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